3H1D
Structure of the HUWE1 HECT Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.654, 55.653, 69.585 |
| Unit cell angles | 90.00, 122.49, 90.00 |
Refinement procedure
| Resolution | 29.347 - 1.892 |
| R-factor | 0.1687 |
| Rwork | 0.166 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nd7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.202 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 30294 | |
| <I/σ(I)> | 17.2 | 2.1 |
| Completeness [%] | 98.3 | 96.3 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | 0.1 M citric acid, 1.8 M (NH4)2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
