3H0M
Structure of trna-dependent amidotransferase gatcab from aquifex aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0274 |
Spacegroup name | P 1 |
Unit cell lengths | 128.248, 129.856, 155.069 |
Unit cell angles | 90.01, 89.96, 90.11 |
Refinement procedure
Resolution | 39.370 - 2.800 |
R-factor | 0.25623 |
Rwork | 0.254 |
R-free | 0.30540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h0l |
RMSD bond length | 0.013 |
RMSD bond angle | 1.491 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.400 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.061 | 0.478 |
Number of reflections | 241473 | |
<I/σ(I)> | 11.5 | 1.6 |
Completeness [%] | 98.0 | 96.8 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1:1 ratio of Protein solution (6-9mg/ml GatCAB,10mM HEPES, 50uM Zn acetate,10mM Gln, 0.6mM ATP) mix with well solution (10-12% PEG3350, 10mM Mg formate), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |