3H0G
RNA Polymerase II from Schizosaccharomyces pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 163.034, 202.684, 391.258 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.695 - 3.650 |
| R-factor | 0.297 |
| Rwork | 0.297 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wcm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.117 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.850 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmerge | 0.085 | 0.600 |
| Total number of observations | 45357 | |
| Number of reflections | 138872 | |
| <I/σ(I)> | 6.702 | 1.3 |
| Completeness [%] | 96.6 | 95.2 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 289 | 50mM MES pH 6.3, 300mM ammonium acetate, 5-6.5% (w/v) PEG 4000, 10mM DTT, 7.1mM C8E5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
