3H00
Structure of the C-terminal Domain of a Putative HIV-1 gp41 Fusion Intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.764, 47.499, 74.824 |
| Unit cell angles | 90.00, 128.32, 90.00 |
Refinement procedure
| Resolution | 40.100 - 2.200 |
| R-factor | 0.21403 |
| Rwork | 0.212 |
| R-free | 0.25526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gwo |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.406 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.100 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.044 | 0.269 |
| Number of reflections | 16151 | |
| <I/σ(I)> | 18.7 | 3.1 |
| Completeness [%] | 93.7 | 76.2 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 295 | 3% PEG8000, 0.1M Tris-HCl, 0.1M cesium chloride, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
