3GZE
Algal prolyl 4-hydroxylase complexed with zinc and (Ser-Pro)5 peptide substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.932 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.250, 58.750, 105.160 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 20.600 - 1.980 |
| R-factor | 0.21888 |
| Rwork | 0.217 |
| R-free | 0.25392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v4a |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.813 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.040 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.062 | 0.201 |
| Number of reflections | 68688 | |
| <I/σ(I)> | 12.16 | 4.3 |
| Completeness [%] | 93.4 | 87.1 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under-oil | 5.5 | 295 | 5% PEG Smear, 0.1M acetate, 10mM zinc acetate, pH 5.5, Microbatch under-oil, temperature 295K |
