3GYW
Crystal structure of nucleosome assembly protein from Plasmodium falciparum at 2.4 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.240, 37.870, 79.140 |
| Unit cell angles | 90.00, 99.70, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| Rwork | 0.220 |
| R-free | 0.26700 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.981 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.040 | 0.206 |
| Number of reflections | 8943 | |
| <I/σ(I)> | 37.6 | 8.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 20% PEG 3350, 0.2M magnesium chloride, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
