3GYS
Crystal structure determination of cat (Felis silvestris catus) hemoglobin at 2.9 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5417 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.022, 96.465, 146.345 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.130 - 2.900 |
| R-factor | 0.19562 |
| Rwork | 0.187 |
| R-free | 0.27106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d4x |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.583 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 23886 | |
| <I/σ(I)> | 4.1 | 1.4 |
| Completeness [%] | 96.0 | 98.5 |
| Redundancy | 3.65 | 3.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 40% PEG 3350, 50mM phosphate buffer at pH 6.7, 1M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
