3GYE
Didydroorotate dehydrogenase from Leishmania major
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 61 |
| Unit cell lengths | 143.664, 143.664, 69.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.500 - 2.000 |
| R-factor | 0.17018 |
| Rwork | 0.169 |
| R-free | 0.19962 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dor |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.202 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 124.035 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.071 |
| Number of reflections | 55526 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 100.0 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M Sodium Tribasic Dihydrate pH 5.6, 1M Lithium Sulfate and 0.5M amonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
