3GXQ

Structure of ArtA and DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11588
Spacegroup name	P 1 21 1
Unit cell lengths	37.830, 61.130, 40.500
Unit cell angles	90.00, 117.32, 90.00

Refinement procedure

Resolution	35.980 - 2.350
R-factor	0.237
Rwork	0.237
R-free	0.26100
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.080	2.090
High resolution limit [Å]	1.980	1.980
Rmerge	0.074	0.258
Number of reflections	6644
<I/σ(I)>	11.5	2.3
Completeness [%]	93.1	73.7
Redundancy	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	10% PEG 2000, 0.1M Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 2000
2	1	1	Acetate
3	1	2	PEG 2000
4	1	2	Acetate