3GXB

Crystal structure of VWF A2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	55.110, 60.810, 56.350
Unit cell angles	90.00, 99.09, 90.00

Refinement procedure

Resolution	27.820 - 1.900
R-factor	0.185
Rwork	0.183
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fe8
RMSD bond length	0.006
RMSD bond angle	1.010
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.820	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.104	0.801
Number of reflections	28792
<I/σ(I)>		2
Completeness [%]	98.8	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.2	277	0.1 M Bis-Tris pH 7.2, 0.2 M lithium sulfate, 25 % PEG3350, vapor diffusion, hanging drop, temperature 277K