3GX0

Crystal Structure of GSH-dependent Disulfide bond Oxidoreductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-01
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	68.490, 68.490, 111.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.1935
Rwork	0.191
R-free	0.24981
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.662
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.130	0.551
Number of reflections	12571
<I/σ(I)>	17.5	3.35
Completeness [%]	99.8	98.5
Redundancy	10.9	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			Protein in 20 mM potassium dihydrogen phosphate, 2 mM DTT, 1mM EDTA, 10 mM glutathione. Well solution: 20% w/v PEG 3000, 100 mM acetate., VAPOR DIFFUSION, HANGING DROP