3GWO
Structure of the C-terminal Domain of a Putative HIV-1 gp41 Fusion Intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2003-07-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793,0.9796,0.9681 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 33.957, 33.957, 174.838 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.700 - 1.650 |
| R-factor | 0.23288 |
| Rwork | 0.232 |
| R-free | 0.25930 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.722 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.700 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.062 | 0.485 |
| Number of reflections | 14912 | |
| <I/σ(I)> | 15.6 | 5.2 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 8.4 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 28% Jeffamine M600, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
