3GVX
Crystal structure of Glycerate dehydrogenase related protein from Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-23 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.512, 77.842, 152.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.420 - 2.200 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.26100 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Rmerge | 0.094 | 0.490 |
| Number of reflections | 38525 | |
| <I/σ(I)> | 14.8 | 11 |
| Completeness [%] | 94.4 | 71.8 |
| Redundancy | 8.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 298 | 0.2M Pottasium phosphate dibasic, 25% peg 3350, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
