3GVV

Single-chain UROD Y164G (GY) mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-08-18
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	102.931, 102.931, 72.148
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.690 - 2.800
R-factor	0.181
Rwork	0.172
R-free	0.26200
RMSD bond length	0.017
RMSD bond angle	1.672
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.183	0.182	0.455
Number of reflections	10977
<I/σ(I)>	13.742
Completeness [%]	98.1	100	85.9
Redundancy	12	14.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop			hanging drop