3GVT

Structure and RNA binding of the mouse Pumilio-2 Puf Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.92
Spacegroup name	P 63
Unit cell lengths	150.540, 150.540, 77.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.870 - 2.800
R-factor	0.19457
Rwork	0.192
R-free	0.25317
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gvo
RMSD bond length	0.013
RMSD bond angle	1.328
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.880	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.089	0.471
Number of reflections	24746
<I/σ(I)>	11.5	5.7
Completeness [%]	99.9	100
Redundancy	5.4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2M ammonium citrate, 0.2M Li2SO4, 22%(w/v) PEG 4000 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K