3GV6
Crystal Structure of human chromobox homolog 6 (CBX6) with H3K9 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.075, 40.454, 53.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.850 - 1.760 |
| R-factor | 0.21354 |
| Rwork | 0.210 |
| R-free | 0.26634 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dm1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.390 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.080 | 0.473 |
| Number of reflections | 6339 | |
| <I/σ(I)> | 10.2 | 1.66 |
| Completeness [%] | 98.8 | 89.2 |
| Redundancy | 6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 14% PEG 3350, 0.2M MgCl2, 0.1M HEPES, pH7.5 , VAPOR DIFFUSION, SITTING DROP, temperature 297K |
