3GV4
Crystal structure of human HDAC6 zinc finger domain and ubiquitin C-terminal peptide RLRGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.657, 36.093, 88.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.450 - 1.720 |
| R-factor | 0.17695 |
| Rwork | 0.174 |
| R-free | 0.23034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c5k |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.191 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.073 | 0.231 |
| Number of reflections | 10865 | |
| <I/σ(I)> | 14.1 | 4.26 |
| Completeness [%] | 94.5 | 72.5 |
| Redundancy | 5.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 20% PEG3350, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
