3GV0
Crystal structure of LacI family transcription regulator from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.710, 86.710, 111.224 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.360 - 2.350 |
| R-factor | 0.20113 |
| Rwork | 0.198 |
| R-free | 0.25450 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.992 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.360 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.124 | 0.423 |
| Number of reflections | 20658 | |
| <I/σ(I)> | 15.8 | 4.8 |
| Completeness [%] | 99.8 | 97.6 |
| Redundancy | 18.9 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES pH 7.5, 20% PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
