3GUC
Human Ubiquitin-activating Enzyme 5 in Complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-18 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 77.394, 77.394, 205.892 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.820 - 2.250 |
| R-factor | 0.20562 |
| Rwork | 0.203 |
| R-free | 0.25485 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zfn |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.531 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 34922 | |
| <I/σ(I)> | 23.878 | 2.89 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 287 | 1 M LITHIUM SULPHATE, 0.3 M AMMONIUM SULPHATE,0.1 M SODIUM CITRATE, PH 6.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 287K |
