3GU0
Promiscuous Substrate Recognition in Folding and Assembly Activities of the Trigger Factor Chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97903 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.387, 114.487, 94.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.500 |
| R-factor | 0.25 |
| Rwork | 0.244 |
| R-free | 0.35800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.226 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 3.570 |
| High resolution limit [Å] | 3.450 | 7.320 | 3.450 |
| Rmerge | 0.081 | 0.041 | 0.321 |
| Number of reflections | 6747 | ||
| <I/σ(I)> | 17.492 | ||
| Completeness [%] | 93.7 | 88.9 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 10-15% PEG4000, 200 MgCl2, 100 Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
