3GTY
Promiscuous Substrate Recognition in Folding and Assembly Activities of the Trigger Factor Chaperone
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792,0.9794,0.9687 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.162, 94.162, 193.072 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.400 |
| R-factor | 0.261 |
| Rwork | 0.257 |
| R-free | 0.32900 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.321 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.06) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
| Rmerge | 0.057 | 0.022 | 0.369 |
| Number of reflections | 13240 | ||
| <I/σ(I)> | 21.369 | ||
| Completeness [%] | 98.4 | 88.9 | 99.9 |
| Redundancy | 3.3 | 3.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 5-10 % PEG4000, 100 NaOAc, pH 5.5, vapor diffusion, hanging drop, temperature 298K |
