3GTT
Mouse SOD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-18 |
| Detector | RIGAKU RAXIS VII |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 112.454, 194.495, 149.841 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.760 - 2.400 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vr7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.076 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 64203 | |
| <I/σ(I)> | 11.5 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 0.1 M Tris, 30% PEG 1000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
