3GSZ
Structure of the genotype 2B HCV polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.251, 64.497, 135.583 |
| Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.900 |
| R-factor | 0.16868 |
| Rwork | 0.166 |
| R-free | 0.21006 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NS5B genotype 2a |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.870 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 104470 | |
| <I/σ(I)> | 3 | |
| Completeness [%] | 97.3 | 87 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 300 | 6.5mg/ml enzyme, 10% PEG4K, 100mM NaCitrate, 200mM NaCl, 5mM DTT , pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
