3GRN
CRYSTAL STRUCTURE OF MUTT PROTEIN FROM Methanosarcina mazei Go1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-19 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.397, 42.437, 55.599 |
| Unit cell angles | 99.98, 100.43, 117.57 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.20804 |
| Rwork | 0.207 |
| R-free | 0.25338 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 | 0.790 |
| Number of reflections | 32938 | |
| <I/σ(I)> | 7.6 | 1.2 |
| Completeness [%] | 97.2 | 95.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.8 | 294 | 100MM SODIUM ACETATE PH 4.8, 27% PEG4K, 200MM AMMONIUM SULFATE, 10% GLYCEROL, VAPOR DIFFUSION, TEMPERATURE 294K |
