3GQY
Activator-Bound Structure of Human Pyruvate Kinase M2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-01-10 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.149, 152.952, 93.159 |
Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.850 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zjh |
RMSD bond length | 0.011 |
RMSD bond angle | 1.014 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.980 | 1.850 |
Rmerge | 0.067 | 0.030 | 0.969 |
Number of reflections | 182520 | ||
<I/σ(I)> | 12.3 | ||
Completeness [%] | 96.9 | 99.4 | 94.3 |
Redundancy | 3.8 | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 20% PEG3350, 0.2M diammonium tartrate, 0.005M activator, 0.005M ATP, vapor diffusion, temperature 291K |