3GQJ
Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 59.130, 59.130, 293.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.580 - 1.850 |
| R-factor | 0.22819 |
| Rwork | 0.226 |
| R-free | 0.27360 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.254 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.960 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 26540 | |
| <I/σ(I)> | 19.3 | 4.4 |
| Completeness [%] | 97.2 | 88.1 |
| Redundancy | 12 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 100 mM tri-Sodium Citrate pH 6.2, 2.45 M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
