3GQE
Crystal structure of macro domain of Venezuelan Equine Encephalitis virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9749 |
| Spacegroup name | I 4 |
| Unit cell lengths | 129.610, 129.610, 42.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.260 - 2.300 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gpg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.348 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.269 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.139 | 0.264 |
| Number of reflections | 15851 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG 3350, 11mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
