3GQ9

Crystal Structure of the Bacteriophage phi29 gene product 12 N-terminal fragment in an apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-21
Detector	MAR CCD 165 mm
Wavelength(s)	0.99
Spacegroup name	H 3 2
Unit cell lengths	89.633, 89.633, 585.719
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.690 - 2.000
R-factor	0.174
Rwork	0.172
R-free	0.20400
RMSD bond length	0.010
RMSD bond angle	1.262
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.690	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.079	0.039	0.397
Number of reflections	61778
<I/σ(I)>	24.302
Completeness [%]	99.4	99.5	98.7
Redundancy	7	7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	298	100mM CHES at ~pH 10.0 and 8% PEG3K. Crystalls obtained were then soaked in a mother liquid containing 10mM EDTA for ~5 hours., VAPOR DIFFUSION, HANGING DROP, temperature 298K