3GPE
Crystal Structure Analysis of PKC (alpha)-C2 domain complexed with Ca2+ and PtdIns(4,5)P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 2007-09-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.848, 57.848, 90.479 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.24434 |
| Rwork | 0.243 |
| R-free | 0.27730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | C2 DOMAIN FROM PROTEIN KINASE C (ALPHA) COMPLEXED WITH CA2+ AND PHOSPHATIDYLSERINE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.707 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 14140 | |
| <I/σ(I)> | 13.3 | 1.6 |
| Completeness [%] | 99.0 | 97.3 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 8000, 50mM potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
