3GP9
Crystal structure of the Mimivirus NDK complexed with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-07-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.064 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.482, 152.951, 183.858 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.527 - 1.800 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b8q |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.993 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.500 | 2.000 |
| High resolution limit [Å] | 1.800 | 1.900 |
| Number of reflections | 102912 | |
| <I/σ(I)> | 6.4 | 3.2 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 40 to 44% MPD, 0.1M MOPS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
