3GOX
Crystal structure of the beta-beta-alpha-Me type II restriction endonuclease Hpy99I in the absence of EDTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91841 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 90.682, 90.682, 334.933 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.600 - 1.500 |
| R-factor | 0.16964 |
| Rwork | 0.169 |
| R-free | 0.18639 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.207 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 84393 | |
| <I/σ(I)> | 16.62 | 3.9 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.6 | 3.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M HEPES/Sodium hydroxide pH 7.5, 0.1 M Sodium chloride, 30 % PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES/Sodium hydroxide | ||
| 2 | 1 | 1 | Sodium chloride | ||
| 3 | 1 | 1 | PEG 400 | ||
| 4 | 1 | 2 | HEPES/Sodium hydroxide | ||
| 5 | 1 | 2 | Sodium chloride | ||
| 6 | 1 | 2 | PEG 400 |
