3GOI

Human glucokinase in complex with a synthetic activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	2001-10-02
Detector	WEISSENBERG
Wavelength(s)	1.000
Spacegroup name	P 65 2 2
Unit cell lengths	80.085, 80.085, 323.974
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.350 - 2.520
R-factor	0.209
Rwork	0.205
R-free	0.28200
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.030
RMSD bond angle	2.563
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.610
High resolution limit [Å]	2.520	5.430	2.520
Rmerge	0.047	0.029	0.143
Number of reflections	21669
<I/σ(I)>	71.222
Completeness [%]	98.3	99.5	91.1
Redundancy	20.3	17.4	18.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	298	30% PEG 1500, HEPES, pH6.6, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP