3GOF
Calmodulin bound to peptide from macrophage nitric oxide synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 180 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.923, 57.773, 60.231 |
| Unit cell angles | 90.00, 93.06, 90.00 |
Refinement procedure
| Resolution | 9.340 - 1.450 |
| R-factor | 0.14802 |
| Rwork | 0.145 |
| R-free | 0.20822 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.565 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.520 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 52623 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 97.0 | 95.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.6, 20% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
