3GOC

Crystal structure of the Endonuclease V (SAV1684) from Streptomyces avermitilis. Northeast Structural Genomics Consortium Target SvR196

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.97853
Spacegroup name	C 1 2 1
Unit cell lengths	123.742, 40.563, 107.422
Unit cell angles	90.00, 121.61, 90.00

Refinement procedure

Resolution	19.830 - 1.600
R-factor	0.178
Rwork	0.177
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ga2
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	COMO
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.043	0.103
Number of reflections	112856
<I/σ(I)>	24.8	8.86
Completeness [%]	98.0	98
Redundancy	2.8	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291	Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM Sodium chloride, 5 mM DTT. Reservoir solution: 100 mM Bis-Tris pH 7.2, 20% PEG 3350, 200 mM Magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K