3GO8
MutM encountering an intrahelical 8-oxoguanine (oxoG) lesion in EC3-loop deletion complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.244, 92.655, 105.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.380 - 1.890 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.21800 |
| Starting model (for MR) | 2F5O protein part only |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.347 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.960 |
| High resolution limit [Å] | 1.890 | 4.070 | 1.890 |
| Rmerge | 0.101 | 0.063 | 0.490 |
| Number of reflections | 35790 | ||
| <I/σ(I)> | 15.897 | ||
| Completeness [%] | 98.7 | 93.4 | 100 |
| Redundancy | 5.6 | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8K, sodium cacodylate, glycerol, pH 7.0, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8K | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 2 | PEG 8K | ||
| 5 | 1 | 2 | sodium cacodylate | ||
| 6 | 1 | 2 | glycerol |
