3GO7

Crystal Structure of M. tuberculosis ribokinase (Rv2436) in complex with ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	79.025, 119.207, 89.768
Unit cell angles	90.00, 115.00, 90.00

Refinement procedure

Resolution	42.220 - 2.500
R-factor	0.179
Rwork	0.176
R-free	0.23700
RMSD bond length	0.013
RMSD bond angle	1.399
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.063	0.019	0.438
Number of reflections	26190
<I/σ(I)>	19.959
Completeness [%]	99.1	99.6	89.6
Redundancy	3.6	3.7	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	295	0.2 M Sodium/potassium phosphate, 0.1 M Bis-Tris propane, 16% PEG 3350, pH 6.5, vapor diffusion, temperature 295K