3GNZ
Toxin fold for microbial attack and plant defense
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0052 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 69.630, 140.900, 49.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.400 - 1.350 |
| R-factor | 0.15694 |
| Rwork | 0.156 |
| R-free | 0.18363 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gnu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.218 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.450 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.050 | 0.281 |
| Number of reflections | 53077 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 98.3 | 92.1 |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1M Tris HCl pH 9.0, 20% PEG 4000, 0.5M MgCl2, 5% methanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
