3GNQ
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase, type I from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Collection date | 2008-12-03 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.932, 173.797, 93.501 |
| Unit cell angles | 90.00, 93.29, 90.00 |
Refinement procedure
| Resolution | 48.840 - 2.400 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.26300 |
| Structure solution method | 1obf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.299 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.096 | 0.540 |
| Number of reflections | 112883 | |
| <I/σ(I)> | 13.554 | 1.74 |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 3.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | Molecular Dimensions PACT Premier screen condition E1, 25% PEG 3350, 0.2 M NaF with 25% glycerol as cryo-protectant, 33.4 mg/mL protein, crystal ID 201195e1, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
