3GN4
Myosin lever arm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-29 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.790, 118.390, 182.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.515 - 2.700 |
| R-factor | 0.2111 |
| Rwork | 0.208 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bkh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.961 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.630 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.077 | 0.447 |
| Number of reflections | 31647 | |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 277 | 16 % PEG 2K, 100 mM MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
