3GMY
Crystal Structure of Beta-Lactamse Inhibitory Protein-Like Protein (BLP), Selenomethionine Derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-27 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.167, 67.195, 41.208 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.000 - 1.700 |
| R-factor | 0.20328 |
| Rwork | 0.200 |
| R-free | 0.27300 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.090 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.990 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 30474 | |
| <I/σ(I)> | 34.3 | |
| Completeness [%] | 96.5 | 96.2 |
| Redundancy | 7.6 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8.5 | 294 | 11 mg/ml protein in 30 mM tris, 150 mM NaCl mixed 1:1 with well solution 30% PEG 4000, 0.2 M sodium acetate trihydrate, pH 8.5, hanging drop, temperature 294K |
