3GMX
Crystal Structure of Beta-Lactamse Inhibitory Protein-Like Protein (BLP) at 1.05 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-28 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 1.11588 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.771, 99.925, 41.643 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 49.900 - 1.050 |
| R-factor | 0.126 |
| Rwork | 0.125 |
| R-free | 0.15400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.533 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.940 | 1.077 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.265 | |
| Number of reflections | 114297 | |
| <I/σ(I)> | 6.5 | 2.3 |
| Completeness [%] | 86.1 | 65.9 |
| Redundancy | 9.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 6.5 | 294 | starting concentration as follows: 5.5 mg/ml protein, 10% PEG 8000, 0.1 M magnesium acetate tetrahydrate, 50 mM sodium cacodylate, 75 mM NaCl, 15 mM tris, pH 6.5, microbatch under oil, temperature 294K |
