3GMV
Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Apo Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.735, 44.071, 45.291 |
| Unit cell angles | 90.00, 104.58, 90.00 |
Refinement procedure
| Resolution | 20.490 - 1.800 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.22695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 17522 | |
| <I/σ(I)> | 28.336 | 11.6 |
| Completeness [%] | 91.0 | 68.7 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor batch | 294 | 20 mg/ml protein in 50 mM tris, pH 8.0, 50 mM NaCl mixed 1:1 with well solution 30% PEG 4000, 0.3 M lithium chloride, 0.1 M TrisCl, pH 8.5, hanging drop vapor batch, temperature 294K |
