3GMI
Crystal structure of a protein of unknown function from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-17 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.935, 73.988, 85.024 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.810 - 1.910 |
| R-factor | 0.1756 |
| Rwork | 0.174 |
| R-free | 0.20858 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.643 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.810 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.116 | 0.660 |
| Number of reflections | 33426 | |
| <I/σ(I)> | 17.3 | 1.27 |
| Completeness [%] | 99.1 | 89.71 |
| Redundancy | 8.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M Lithium chloride, 0.05M Magnesium sulfate, 8% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
