3GM0
Anti-methamphetamine single chain Fv in complex with MDMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.720, 64.806, 48.322 |
| Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| Rwork | 0.214 |
| R-free | 0.27100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.337 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 2.580 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 8316 | |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 3.75 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.3 | 287 | 1.15M sodium Citrate, 0.28M Imidazole malate, pH 8.3, VAPOR DIFFUSION, temperature 287K |
