3GLV
Crystal structure of the lipopolysaccharide core biosynthesis protein from Thermoplasma volcanium GSS1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.391, 67.187, 99.032 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.640 - 1.990 |
| R-factor | 0.19694 |
| Rwork | 0.195 |
| R-free | 0.24228 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.733 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.640 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.107 | 0.644 |
| Number of reflections | 26291 | |
| <I/σ(I)> | 29.67 | 2.3 |
| Completeness [%] | 94.9 | 69.61 |
| Redundancy | 8.5 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M Calcium chloride, 0.1M Tris-HCl, 25% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
