3GLD
Crystal Structure of the Major Pilin from Streptococcus pyogenes E117A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.307, 52.149, 127.316 |
| Unit cell angles | 90.00, 98.83, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.030 |
| R-factor | 0.21487 |
| Rwork | 0.212 |
| R-free | 0.26471 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b2m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.303 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 54403 | |
| <I/σ(I)> | 32.6 | 5.8 |
| Completeness [%] | 95.8 | 84.9 |
| Redundancy | 5.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.2M Potassium acetate, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |
