3GKY
The Structural Basis of an ER Stress-Associated Bottleneck in a Protein Folding Landscape
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-11 |
| Wavelength(s) | 0.948 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.225, 78.225, 36.712 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.580 - 1.800 |
| Rwork | 0.209 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1trz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.580 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.182 |
| Number of reflections | 7299 | |
| <I/σ(I)> | 16.5 | 7.4 |
| Completeness [%] | 94.3 | 90.1 |
| Redundancy | 6.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 0.02 M Tris, 0.05 M sodium citrate, 5% acetone, 0.03% phenol, 0.01% zinc acetate, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
