3GJE

Rational development of high-affinity T-cell receptor-like antibodies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 43
Unit cell lengths	117.057, 117.057, 78.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.300
R-factor	0.22628
Rwork	0.222
R-free	0.30231
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rzf
RMSD bond length	0.021
RMSD bond angle	2.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Number of reflections	44489

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			VAPOR DIFFUSION, SITTING DROP