3GIA
Crystal Structure of ApcT Transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.482, 99.499, 72.693 |
| Unit cell angles | 90.00, 117.71, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.320 |
| R-factor | 0.20405 |
| Rwork | 0.203 |
| R-free | 0.23274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gi9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.106 | 0.715 |
| Number of reflections | 30472 | |
| <I/σ(I)> | 18.6 | 2.1 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 7.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 9 | 293 | 0.1M Bicine, 0.1M sodium chloride, 39-45% PEG 555 MME, pH 9.0, Microbatch, temperature 293K |
