3GHQ
Crystal Structure of E. coli W35F BFR mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.7429 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 207.641, 207.641, 142.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.610 - 2.700 |
| R-factor | 0.24419 |
| Rwork | 0.243 |
| R-free | 0.26169 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e1j |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.554 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 81605 | |
| <I/σ(I)> | 17.2 | 3.8 |
| Completeness [%] | 95.6 | 76.8 |
| Redundancy | 10.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 289 | 2.45M Ammonium Sulfate, 0.1M sodium Citrate pH 5.0, temperature 289K |
